Recombinant Human Growth Hormone

Recombinant Human Growth Hormone (rhGH) is a bioengineered protein that is identical to the growth hormone naturally produced by the human pituitary gland. It plays a crucial role in cell regeneration, growth, and maintaining healthy human tissue, including the brain and various vital organs. Produced through recombinant DNA technology, rhGH is used in medicine to treat growth disorders in children and growth hormone deficiency in adults. Despite its complex nature, rhGH has been successfully used in the healthcare industry, demonstrating the power of biotechnology.

Transfer of the end-to-end technology available in GMP-like environment.

Recombinant human growth hormone (rhGH) is produced in Pichia pastoris using
an expression vector pPIC9K (Cat. No. V175-20, Invitrogen, CA) driven by the AOX promoter and induced by methanol.
Production: 10L fermententation (4 day methanol induction) yields around 10 g of human growth hormone.
The growth hormone is purified from the culture supernatant by cation exchange chromatography and further hydrophobic interaction chromatography. Final yield of the process is at least 20%.

GH stimulates growth, cell reproduction, and cell regeneration. In children, GH deficiencies can cause short stature, growth failure, and delayed sexual maturity. Adult GH deficiency presents with reduced lean body mass, increased adiposity, reduced muscle strength, and ultimately premature mortality.
GH replacement therapy is used to treat many growth disorders, including Turner syndrome, chronic renal failure, and Prader–Willi syndrome.